In vitro assay for osteoinductive activity of diff

Purpose: Various bone graft materials have been used for periodontal tissue regeneration. Demineralized freeze-dried bone allograft (DFDBA) is a widely used bone substitute. The current widespread use of DFDBA is based on its potential osteoinductive ability. Due to the lack of verifiable data, the purpose of this study was to assess the osteoinductive activity of different DFDBAs in vitro. Methods: Sarcoma osteogenic (SaOS-2) cells (human osteoblast-like cells) were exposed to 8 mg/mL and 16 mg/mL concentrations of three commercial types of DFDBA: Osseo+, AlloOss, and Cenobone. The effect of these materials on cell proliferation was determined using the 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide assay. The osteoinductive ability was evaluated using alizarin red staining, and the results were confirmed by evaluating osteogenic gene expression using reverse transcription polymerase chain reaction (RT-PCR). Results: In the SaOS-2 cells, an 8 mg/mL concentration of Osseo+ and Cenobone significantly increased cell proliferation in 48 hours after exposure (P<0.001); however, in these two bone materials, the proliferation of cells was significantly decreased after 48 hours of exposure with a 16 mg/mL concentration (P<0.001). The alizarin red staining results demonstrated that the 16 mg/mL concentration of all three tested DFDBA induced complete morphologic differentiation and mineralized nodule production of the SaOS-2 cells. The RT-PCR results revealed osteopontin gene expression at a 16 mg/mL concentration of all three test groups, but not at an 8 mg/mL concentration. Conclusions: These commercial types of DFDBA are capable of decreasing proliferation and increasing osteogenic differentiation of the SaOS-2 cell line and have osteoinductive activity in vitro.

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Comparison of bone regeneration using three demine

Background: It has been stated that the bone allografts from different tissue banks may lead to various amount of bone induction, so the aim of this study was to evaluate bone regeneration of three demineralized allografts both histologically and histomorphometrically in rabbits calvaria bone defects.Materials and Methods: In this double-blind randomized experimental animal study, 32 critical size defects (11-mm diameter) in the calvaria of 16 male New Zealand white rabbits were randomly fi lled with three demineralized freeze-dried bone allografts (DBM, CENOBONE, DEMBONE), while the nongrafted defect was regarded as control group. After 6 and 12 weeks of healing, the experimental animals were euthanized for specimen preparation. After histological evaluation, histomorphometric analysis was performed to quantify new bone formation and remained graft particles. The data were analyzed by one-way ANOVA with Tukey’s ad-hoc test and t-test. (P < 0.05 was considered to be statistically signifi cant).

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A Comparision of Two Types of Decalcified Freeze-D

Background: Decalcified freeze-dried bone allograft (DFDBA) may have the potential to enhance bone formation around dental implants. Our aim in this study was the evaluation and comparison of two types of DFDBA in treatment of dehiscence defects around Euroteknika® implants in dogs. Methods:In this prospective clinical trial animal study, all mandibular premolars of three Iranian dogs were extracted. After 3 months of healing, fifteen SLA type Euroteknika® dental implants (Natea) with 4.1mm diameter and 10mm length were placed in osteotomy sites with dehiscence defects of 5mm length, 4 mm width, and 3mm depth. Guided bone regeneration (GBR) procedures were performed using Cenobone and collagen membrane for six implants, the other six implants received Dembone and collagen membrane and the final three implants received only collagen membrane. All implants were submerged. After 4 months of healing, implants were uncovered and stability (Implant Stability Quotient) of all implants was measured. Then, block biopsies of each implant site were taken and processed for ground sectioning and histomorphometric analysis. The data was analyzed by ANOVA and Pearson tests. P value less than 0.05 was considered to be significant.

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Determination of orthodontic tooth movement and ti

Background: Socket preservation after tooth extraction is one of the indications of bone grafting to enhance preorthodontic condition. The aim of this study is to determine the effects of socket preservation on the immediate tooth movement, alveolar ridge height preservation and orthodontic root resorption. Materials and Methods: In a split-mouth technique, twelve sites in three dogs were investigated as an experimental study. Crushed demineralized freeze-dried bone allograft (DFDBA) (CenoBone®) was used as the graft material. The defects were made by the extraction of 3rd premolar. On one side of each jaw, the defects were preserved by DFDBA and defects of the other side left opened as the control group. Simultaneously the teeth adjacent to the defects were pulled together by a NiTi coil spring. After eight weeks, the amount of (OTM), alveolar height, and root resorption were measured. Analysis of variance was used for purpose of comparison.

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Ridge preservation with OSSEO+ compared to Cenobon

Abstract: Alveolar ridge reduction after tooth extraction could be minimized via ridge preservation by using grafting material. The aim of this study was to compare OSSEO+ (allograft, made by IMTEC Corporation) with CenoBone (allograft, made by Tissue Regeneration Corporation) in preservation and bone formation of the alveolar ridge after tooth extraction.

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Efficacy of Acellular Dermal Matrix Allograft (Cen

Purpose: This study sought to assess the clinical efficacy of CenoDerm® acellular dermal matrix allograft (ADMA) with and without plasma rich in growth factors (PRGF) for gingival augmentation. Methods: This split-mouth double-blind randomized controlled clinical trial was conducted on 10 patients requiring augmentation of keratinized gingiva bilaterally in the mandible (<2mm). The recipient sites were prepared using the conventional free gingival graft (FGG) technique. CenoDerm® was rehydrated with PRGF and saline solution in the test and control groups, respectively. Keratinized tissue (KT) width, gingival thickness, wound healing (healing index by Landry et al), and pain scores according to the visual analog scale (VAS) were evaluated and compared at baseline (preoperatively) and at 2 weeks, 1 month and 3 months, post-operatively. Data were analyzed using the paired t-test and the Wilcoxon signed tank test. Results: At 3 months, KT width was significantly greater in the test compared to the control group (P<0.0001). No significant differences were found between the two groups regarding the healing index at any time point post-operatively. Pain scores were significantly lower in the control group at 1 (P<0.01) and 2 (P<0.009) days; but the difference in this regard at day 3 was not significant. Gingival thickness was not significantly different between the two groups at any time point. Conclusion: Both ADMA+PRGF and ADMA only result in favorable clinical outcomes, but the added benefit of PRGF is not ev¬ident. (This study has been registered in http://www.irct.ir/ and registration code is: IRCT201503261760N38)

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Clinical, Histologic and Histomorphometric Evaluat

Objective: Alveolar ridge preservation in patients with inadequate bone volume is one treatment option for successful implant placement and can be done by using bone graft materials. On the other hand, Ceno Bone has been recently produced by Hamanand Saz Baft Kish Co. as a bone bioimplant of allograft origin. This study aimed to assess the clinical, histologic and histomorphometric results of Bone Strip Allograft (CenoBone) for horizontal alveolar ridge augmentation. Methods: In this semi-experimental clinical trial, 7 areas requiring horizontal ridge augmentation and subsequent implant placement in the maxilla were selected using non-randomized consecutive sampling. Surgeries were mostly performed via the buccal cortical plate of the edentulous ridge. The buccal bone was decorticated, Ceno Bone was fixed by titanium screws, covered with Ceno Membrane (resorbable) and sutured. Buccolingual width of the ridge was measured in stage-one surgery and six months later in stage-two surgery for implant placement. A core biopsy was also taken to assess the trabecular thickness, percentage of new bone formation, percentage of remnant particles, degree of inflammation, foreign body reaction, vitality, bone-biomaterial contact and number of blood vessels by microscopic, histologic and histomorphometric analyses of the slides. The clinical ridge width values in the first- and second-stage surgeries were analyzed using Wilcoxon Signed Rank test. Results: The mean clinical ridge width at 2mm distance from the ridge crest was 2.49 (0.72) mm in the first-stage and 4.79 (0.75) mm in the second-stage surgery. The mean clinical ridge width at 5mm distance from the ridge crest was 3.6 (0.57) mm in the first-stage and 6.3 (1.13) mm in the second-stage surgery. At both sites, application of Ceno Bone significantly increased the clinical ridge width in the second-stage surgery (both ps<0.05). Also, inflammation in most specimens (85.7%) was grade I and no case of foreign body reaction was seen. Bone was vital in all patients. The mean trabecular thickness was 87.96 (38.74)μ. The percentage of new bone formation was 58.43 (26.42%) and the percentage of remnant particles was 4.07% (2.44%). Conclusion: The results of this preliminary study revealed that application of CenoBone stimulates osteogenesis and significantly increases the clinical ridge width at 2 and 5mm distances from the ridge crest for implant placement. Key words: Ceno Bone, Clinical, Horizontal augmentation, Osteogenesis, Ridge, Wi

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Opening wedge high tibial osteotomy using tibial w

High tibial osteotomy has been established as an effective surgical intervention in patients with unicompartment osteoarthritis of the knee associated with varus deformity and abnormal load through the medial compartment. The aims of this study were to report the result of open-wedge osteotomy performed with allograft bone and also to evaluate the postoperative clinical results in a series of patients. There are still little medical literatures regarding the use of an allograft bone transplant in open-wedge osteotomy. 37 consecutive cases that had undergone opening wedge osteotomy using allograft bone were studied. They were followed each 2 months after surgery until 6 month. There were 7 men and 30 women, aged ranging from 16 to 66. All patients were followed 6 months after surgery until clinical and radiographic healing of the osteotomy site. All patients could stand and walk on operated limb 6 months after operation, but 11 of them had still pain after this duration. There were no cases of non-union or osteotomy site collapse associated with the use of allograft. Moreover, no significant complication has been detected in these patients with choosing appropriate patients and performing good surgical technique, and the proximal tibial wedge allograft is a satisfactory choice that provides effective clinical and radiographic bone union

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SAVE THE IRREMEDIABLE TOOTH WITH AID OF HYDROXYAPA

Nowadays saving the irremediable tooth under the old method has low risk success, or in some cases it is impossible. This study suggests a method that could predict a good prognosis after the operation. This case was, a crownless upper right 2nd premolar with a big periapical lesion that extended the periradicular area also, with initial palatal root resorption . Upon examination Chronic Preapical Periodontitis diagnosed. After RCT, the tooth was extracted, curetting and irrigation of the socket was done, fill ¼ of the socket with hydroxyapatite and reimplant the tooth, during the reimplantation adjustment of the crown to root ratio considered as well ,then was splinted to the adjacent teeth for two weeks, Antibiotic was prescribed to the patient for 7 days. Radiographic examination after two years shows that no more lesions are seen in the periapical and periradicular area, there was no mobility, and root resorption was aborted, and the tooth was in healthy condition.

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