Application of an Acellular Dermal Matrix Allograf

Several techniques and materials have been introduced for the treatment of gingival recession defects. This article reports the case of a 43-year old female patient with chief complaint of esthetic problem, presenting multiple gingival recession defects in anterior maxilla. CenoDerm combined with coronally positioned flap was used for management of six teeth with gingival recession. Complete root coverage was achieved in 66.6% of treated sites in one-year follow-up and the patient was satisfied with the esthetic result. The mean root coverage was 86.0% ± 22.3. The mean recession depth reduction and clinical attachment gain were 1.8 ± 0.8 mm and 2.5 ± 0.6 mm, respectively. According to the results obtained in this case, CenoDerm can be applied successfully in treatment of multiple gingival recession defects.

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Use of Lincomycin-Impregnated Demineralized Freeze

Purpose: The aim of the present study was to evaluate the periodontal regenerative capacity of demineralized freeze-dried bone allograft (DFDBA) alone or used with local lincomycin. Materials and Methods: In the present single-blind, randomized, controlled clinical trial, 20 subjects 26 years old or older, requiring extraction of bilateral third molars (M3s), were included. Each subject was randomly assigned to receive either DFDBA or DFDBA plus lincomycin therapy. Within the subjects, 1M3 site was randomly selected to be the experimental site and the contralateral served as the control and was permitted to heal without intervention. The primary variables were changes in the probing depth (PD), clinical alveolar bone levels (ABLs), and radiographic alveolar bone density (ABD) on the distal aspect of second molar between baseline (immediately postoperatively) and 26 weeks postoperatively (T26). Appropriate sample sizes and descriptive, bivariate, and multivariate statistics were computed. Results: For both treatment and control sites, between T0 and T26, statistically significant improvements were seen in the ABLs and ABD (P < .05).Within-subject comparisons showed no significant differences in PD, ABL, or ABD between the treatment and control M3 sites at T0 or T26 (P > .05). Also, no significant differences were found in the PD, ABL, or ABD between the 2 treatment M3 sites at T26 (P > .05). Conclusions: The results of the present study have revealed that the PD, ABL, and ABD improved after M3 removal in subjects 26 years old or older, irrespective of the treatment or control group. Reconstructive procedures (eg, DFDBA with or without lincomycin therapy) did not offer predictable benefits compared with a no-treatment protocol in patients younger than 30 years old.

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The Comparison of Acellular Dermal Matrix Allograf

Aim: The aim of this study is to evaluate the effect of Cenoderm for treatment of gingival recession as a substitute for subepithelial connective tissue graft. Methods: In this controlled clinical trial,18 teeth of 9 subjects with bilateral gingival recession was selected. one side was treated by SCTG and coronally displaced flap as a controlled group and the other side was treated by Cenoderm and coronally displaced flap as the test group. Probing pocket Depth(PPD), clinical attachment level, vertical recession depth, recession width, gingival thickness ، keratinized tissue width and the root coverage percentage were measured in the middle part of the buccal surface, before the surgery and 1, 3, and 6 months after the surgery. Also healing index (landry) and pain and beauty indices (VAS) were under investigation. The results obtained from the study were statistically analyzed GLM repeated measures and paired T- test. Results:According to the results,all parameters were improved except probing pocket depth, but the comparison between the groups was not statistically significant.only root coverage percentage and pain index were less in test group statistically significant. The average percentage of root coverage in the control and test groups were respectively 64.44% 9.4% and 82.01% 16.62% Conclusion: both methods showed enhancement of clinical results. But the use of Cenoderm led to less pain and also less root coverage in comparison with SCTG method.

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بررسی رادیوگرافیک تاثیر ماده آلوگرفت Ceno-Bone در

سابقه و هدف: یکی از مشکلات شایع پس از کشیدن دندان، تحلیل استخوان آلوئول و ناکافی بودن استخوان جهت جاگذاري ایمپلنت Ceno-bone است. در زمینه حفظ استخوان آلوئول مواد پیوندي مانند الو گرفتها کاربرد دارند. هدف از این مطالعه بررسی تاثیر ماده (الوگرفت ساخت داخل) در جلوگیري ار تحلیل استخوان آلوئول به روش بررسی رادیوگرافیک بود. 1388 به بخش جراح ی فک و - مواد و روش ها: مطالعه به روش تجربی بر روي 42 ساکت دندانی بیمارانی که در طی سالهاي 1389 انجام شد.تمامی ساکتها split mouth صورت دانشکده دندانپزشکی شاهد مراجعه کرده بودند انجام شد.طراحی این مطالعه به صورت پر شده و بخیه شدند و گروه شاهد بدون اضافه کردن ceno-bone به دو گروه شاهد و مورد تقسیم شدند.ساکتهاي گروه مورد با ماده هیچ ماده اي بخیه شدند. رادیوگرافیها یک هفته، 2 ماه، 6ماه بعد تهیه شدند. تغییرات استخوانسازي بر طبق کتب مرجع بر حسب تجزیه و تحلیل شد. paired-t test میلیمتر اندازهگیري و نتایج توسط 0/86 ±0/ 1 میلیمتر و در گروه مورد 36 /33 ± 0/ یافتهها: میزان تحلیل عمودي استخوان شش ماه پس از جراحی در گروه شاهد 48 در سایر زمانهاي تعیین شده (یک هفته و دو ماه ) بین دو گروه (P<0/ میلیمتر بود و میزان تفاوت در گروه معنیدار بود. ( 002 (P>0/ اختلاف معنیداري دیده نشد. ( 05 یک ماده مناسب جهت بازسازي دیفکتهاي استخوانی م ی باشد و منجر به کاهش ceno-bone نتیجه گیري: به نظر میرسد که تحلیل ساکت پس از کشیدن دندان می شود.

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Histologische Untersuchungen zur Einheilung von Kn

In einem seit 2012 durch Drittmittel der DGZI geförderten Projekt im Labor für Oralbiologische Grundlagenforschung der Poliklinik für Kieferorthopädie an der Zahnklinik der Universität Bonn werden Biopsien aus mit unterschiedlichen Knochenersatzmaterialien augmentierten Bereichen feingeweblich untersucht. Ziel ist die Erforschung der Einheilung dieser Materialien mit histologischen und immunhistochemischen Methoden, um die biologischen Phänomene der Knochenbildung und sie beeinflussender Faktoren zu verstehen. Das Projekt ist auf die Mithilfe der Kolleginnen und Kollegen in den Praxen und Kliniken angewiesen, die aufgefordert sind, für diese Studie Proben aus ihrem eigenen Patientengut zur Verfügung zu stellen.

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CenoDerm vs. Fascia lata for the Prevention of Dor

Abstract Introduction: Dorsal nasal irregularity is a complication of rhinoplasty surgery, mostly seen in patients with thin skin. Acellular dermis (CenoDerm) and homologous fascia lata covering the nasal bone cartilage structure have been used to achieve a smooth surface. In this study, we aimed to investigate clinical outcomes using these two materials. Materials and Methods: After a standard rhinoplasty procedure, a layer of the acellular dermis or homologous fascia lata was placed in the pocket of the dorsum. Patients were evaluated for clinical outcomes at 3, 6, and 12 months after the procedure. Results: Forty-two of 68 patients completed the follow-up period. Patient satisfaction was higher in the homologous fascia lata group. Similarly, nasal dorsum inspection and palpation results were better in the homologous fascia lata group compared with the CenoDerm group but was significant in palpation (P=0.00). There was no complete absorption in the homologous fascia lata group 6 months after surgery (P= 0.04 vs. CenoDerm) but no significant difference was observed at 12 months. Conclusion: Homologous fascia lata is better than acellular dermis in preventing dorsal nasal irregularity after rhinoplasty in thin-skinned patients.

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An Introduction on Tissue Engineering

Abstract Tissue engineering is a science to generate biological substitutes for regeneration, preservation and better operation of damaged tissues. Main components of tissue engineering are scaffolds, cells and growth factors. Also, bioreactors seem to be one of the fundamental parts. Scaffold is a 3D structure as a framework to support cell growth and extra cellular matrix deposition. Cells penetrate to scaffolds and grow, differentiate, proliferate and migrate according to physical and chemical signals. If the environment is appropriate for cells, they deposit ECM and new tissue will be formed. Tissue engineering science is to fabricate scaffolds which are suitable for cellular homing, to create and retain appropriate environment for cell maintenance and to control all the parameters which is important to generate new tissues.

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Ion-Exchange Polymer Nanofibers for Enhanced Osteo

ABSTRACT: Nanofibrous scaffolds with specific modifications have shown promising potential for bone tissue engineering applications. In the present study, poly(ether sulfone) (PES) and sulfonated PES (SPES) nanofibers were fabricated via electrospinning. Calcium ions were then incorporated in SPES by immersion in a Ca(OH)2 solution. The calcium-ion-exchanged SPES (Ca-SPES), PES, and SPES nanofibers were characterized and then evaluated for their osteogenic capacity: both in vitro using stem cell culture and in vivo after subcutaneous implantation in mice. After 7 days of immersion in simulated body fluid, the formation of an apatite layer was only observed on Ca-SPES nanofibers. According to the MTT results, an increasing stem cell population was detected on all scaffolds during the period of study. Using real-time reverse transcriptase−polymerase chain reaction, alkaline phosphatase activity, and calcium content assays, it was demonstrated that the osteogenic differentiation of stem cells was higher on Ca-SPES scaffolds in comparison with PES and SPES nanofibers. Interestingly, Ca-SPES scaffolds were shown to induce ectopic bone formation after 12 weeks of subcutaneous implantation in mice. This was confirmed by mineralization and the production of collagen fibers using van Kossa and Masson’s trichrome staining, respectively. Taken together, it was demonstrated that the incorporation of calcium ions into the ion-exchange nanofibrous scaffolds not only gives them the ability to enhance osteogenic differentiation of stem cells in vitro but also to induce ectopic bone formation in vivo. KEYWORDS: nanofiber, ion-exchange polymer, electrospinning, stem cell, osteogenic differentiation, calcium ion

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A histologic, histomorphometric, and radiographic

Background: Several grafting materials have been used for alveolar ridge augmentation. The literature lacks researches to compare CenoBone to other grafting materials. The aim of this study was to compare CenoBone/CenoMembrane complex to Bio-Oss/Bio-Gide complex in lateral alveolar bone augmentation in terms of radiographic, histologic, and histomorphometric parameters. Materials and Methods: In this randomized controlled trial, ten patients who needed lateral ridge augmentation were selected and augmentations were done using either of CenoBone/ CenoMembrane or Bio-Oss/Bio-Gide complexes. In the re-entry surgery in 6 months following augmentation, core biopsies were taken and clinical, radiographic, histologic, and histomorphometric evaluations were performed. Results: No statistically signifi cant difference was seen between groups except for the number of blood vessels and percentage of residual graft materials. Conclusion: CenoBone seems to present a comparable lateral ridge augmentation to Bio-Oss in.

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