مقاله دکتر خسروی

موضوع مقاله :

بررسی اثرات کاربرد ترکیبی فتو بیو مدولیشن، سلول های مزانشیمی مشتق از چربی انسانی و داربست استخوانی انسانی، بر روند ترمیم نقص استخوانی در سایز بحرانی در کندیل فمور در موش صحرایی نر

Preconditioning adipose-derived stem cells with photobiomodulation significantly increased bone healing in a critical size femoral defect in rats Armin Khosravipour a, Abdollah Amini a, **, Reza Masteri Farahani a, Fatemeh Zare a Atarodsadat Mostafavinia b, Somaye Fallahnezhad c, Saman Akbarzade b, Ava parvandi b, Mehrdad Asgari a, Ahmad Mohammadbeigi d, Fatemehsadat Rezaei e, Seyed Kamran Ghoreishi f, Sufan Chien g, Mohammad Bayat a, g, * a Department of Biology and Anatomical Sciences, Shahid Beheshti University of Medical Sciences, Tehran,Iran b Department of Anatomy, Faculty of Medicine, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran c Department of Anatomy and Cell Biology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran d Department of Radiology, Sina Hospital, Tehran University of Medical Sciences, Tehran, Iran e University of Kentucky College of Pharmacy 789 South Limestone Lexington, Kentucky, 40536, USA f Department of Statistics, Qom University, Qom, Iran g Price Institute of Surgical Research, University of Louisville, and Noveratech LLC, Louisville, KY, USA

a r t i c l e i n f o

Article history: Received 3 July 2020 Accepted 12 July 2020 Available online xxx

Keywords : Critical size bone defect , Fracture healing ,Demineralized bone scaffold, Human adipose-derived stem cell ,Photobiomodulation

a b s t r a c t

We assessed the combined impacts of human demineralized bone matrix (hDBM) scaffold, adiposederived stem cells (hADS), and photobiomodulation (PBM) on bone repair of a critical size femoral defect (CSFD) in 72 rats. The rats were divided into six groups: control (group 1); ADS (group 2 - ADS transplanted into hDBM); PBM (group 3 - PBM-treated CSFDs); ADS þ PBM in vivo (group 4 - ADS transplanted into hDBM and the CSFDs were treated with PBM in vivo); ADS þ PBM in vitro (group 5 - ADS were treated with PBM in vitro, then seeded into hDBM); and ADS þ PBM in vitroþin vivo (group 6 - PBM-treated ADS were seeded into hDBM, and the CSFDs were treated with PBM in vivo. At the anabolic phase (2 weeks after surgery), bone strength parameters of the groups 5, 6, and 4 were statistically greater than the control, ADS, and PBM in vivo groups (all, p ¼ 0.000). Computed tomography (CT) scans during the catabolic phase (6 weeks after surgery) of bone healing revealed that the Hounsfield unit (HU) of CSFD in the groups 2 (p ¼ 0.000) and 5 (p ¼ 0.019) groups were statistically greater than the control group. The groups 5, 4, and 6 had significantly increased bone strength parameters compared with the PBM in vivo, control, and ADS groups (all, p ¼ 0.000). The group 5 was statistically better than the groups 4, and 6 (both, p ¼ 0.000). In vitro preconditioned of hADS with PBM significantly increased  bone repair in a rat model of CSFD in vivo


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